Fig 5. WT and TLR2 KO, but not TLR4 KO, mice were susceptible to the systemic bone deterioration response following repetitive ODE inhalation exposure by micro-CT analysis.

WT, TLR4 KO, and TLR2 KO mice were i.n. treated daily with saline or ODE for 3 weeks whereupon trabecular bone of proximal tibia was analyzed micro-CT analysis. Changes in bone quality and bone quantity were observed in ODE-treated WT mice as compared to saline. TLR4 KO, but not TLR2 KO, animals were generally less responsiveness to bone changes induced by ODE. To compare findings across animal strains, bar graph depicts the mean with SEM bars of the percent change induced by ODE treatments (difference between ODE and saline treatment divided by saline groups multiplied by 100) in bone parameters compiled from three independent studies of 2–3 mice per study (N = 6–9 mice). Asterisks denote statistical significance (*p<0.05) vs. WT.