Fig 7. Inhalant exposure with organic dust extract (ODE) increases osteoclast precursor populations (OCP) in murine bone marrow cells.
WT mice were i.n. treated with saline or ODE daily for three weeks whereupon mice were euthanized and bone marrow cells were collected and analyzed by flow cytometry. After exclusion of debris and dead cells, triple negative (TN) cells were gated based upon CD45R-CD3-CD11blo phenotype with panel A depicting the frequency of TN cells as a percentage of live cells in a bar graph. Panel B depicts a representative dot plot of TN cells expressing CD115 with associated frequency distribution shown in bar graph. Next, representative dot plots with frequency distribution of osteoclast precursor populations: TN CD115+CD117+ (panel C), and TN CD115+CD117+CD27+ (panel D) are shown. All bar graphs represent mean percentage with SEM bars. N = minimum of 6 mice/group from three independent experiments. Statistical significance denoted as asterisks (*p<0.05) vs. saline.