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. 2016 Aug 1;11(8):e0160100. doi: 10.1371/journal.pone.0160100

Fig 2. Gel electrophoresis of fibers and proteins used to quantitate exchange of mutant proteins into the fibers.

Fig 2

Lane 1, 2 myosin; Lane 3,4 RLC-C5 uncut; Lanes 5,6, control unexchanged fibers; lanes 7,8 fibers exchanged with RLC-C44-MSL; lanes 9, 10, fibers exchanged with RLC-C44-MMTS. As can be seen in lanes 7–10 the intensity of the endogenous RLC (LC-2) is diminished with a new band showing the presence of the uncut mutant. Quantitation of the bands showed that the intensity of LC-2 is diminished by 68%, 71%, 73% and 82% in lanes 7–10 respectively. The intensity of the RLC bound to the fibers relative to LC-2 in the controls is 106%, 103%, 96% and 90% in lanes 7–10 respectively. Thus about 70% of the LC-2 in the preparations has been replaced by mutant RLC, with an additional 30% mutant bound non-specifically.