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. 2016 Jul 28;10:1423–1433. doi: 10.2147/OPTH.S105902

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© 2016 Wygladacz and Hook. This work is published and licensed by Dove Medical Press Limited

The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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CLSM images.

Notes: Balafilcon A (A), senofilcon A (B), and samfilcon A (C) incubated overnight with 0.1% (w/v) HA solution, then stained with safranin; images of senofilcon A (D), and samfilcon A (E) after scratching the sorbed HA layer with forceps. HA on balafilcon A could not be imaged by this method, because the stained lens did not sufficiently adhere to the glass slide. A–C Captured using a 20× microscope objective and 2× confocal magnification; D–E were captured using a 4× microscope objective.

Abbreviations: CLSM, confocal laser-scanning microscopy; HA, hyaluronic acid.