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. 2016 Jul 28;10:1423–1433. doi: 10.2147/OPTH.S105902

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© 2016 Wygladacz and Hook. This work is published and licensed by Dove Medical Press Limited

The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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CLSM image of HA distribution over a large senofilcon A lens area.

Notes: The master image of the stained HA network distributed over the entire lens area was prepared by stitching sequential images recorded using a 10× objective. Randomly chosen areas were examined using 20× magnification to visualize the HA-network details. The beads (fiducial marks) were used to localize the image position on the master image.

Abbreviations: CLSM, confocal laser-scanning microscopy; HA, hyaluronic acid.