Figure 2.

Propidium iodide (PI) based analysis of the cell cycle of wildtype and PKD3 deficient MEFs: (A) Wild type MEFs were seeded and starved for 24 h at 50% confluence followed by an incubation with aphidicoline for 24 h and released for another 12 h. Alternatively aphidicoline treated MEFs were incubated with RO-3306 for 24 h and released for 12 h or treated with nocodazole for 24 h before the release (16 h). Individual PI stainings of each step are shown, with each labeled on top of the graph. (B) PKD3 deficient MEFs were treated the same way and analyzed accordingly. (C) Detailed analysis of wild type and PKD3 deficient MEFs after nocodazole treatment. Individual steps are indicated on top of the graphs. For all assays, multiple independent experiments were performed which all showed similar results.