Figure 4.

Cyc17 may be involved in microtubule-chromosome attachment and cohesion resolution, but not spindle formation. (A) β-tubulin staining of the meiotic spindle. (B) Giemsa staining at 5 h shows the kinetochore is stretched by opposing forces in WT but nearly not in cyc17Δ cells. (C) Genes encoding kinetochore components and SAC factors are downregulated in cyc17Δ cells. Heatmap color scheme depicts the log₂(FC) in expression between cyc17Δ and WT at the indicated time points. (D) Diagram showing genes downregulated in cyc17Δ cells in a predicted chromosome segregation network in T. thermophila. Blue, genes downregulated in cyc17Δcells. FC, fold change.