Figure 1.

Flow cytometry analysis of cell cycle in podocytes. Cell cycle phases of undifferentiated (A), differentiated (B) and stimulated (C, 10 ng/ml bFGF) podocytes. After staining with propidium iodide, cells were analyzed by flow cytometry using Flow-Jo software. Under permissive conditions 41.5 % of the cells were found to be in S-phase (A, yellow area). After 12 days under restrictive conditions, the S- phase fraction was reduced to 5.2% (B, yellow area). Stimulation with bFGF were able to rise the S- phase fraction to 10.7% (C, yellow areal). (D) Dose depended effect of bFGF on the cell cycle progression was assessed by determination of S-phase using FlowJo-software.