Figure 2.

AURKA regulates the mitotic modification of SOX2. (A) Mitotic modification of SOX2 was sensitive to AURKA inhibition. PA-1 cells were treated with nocodazole for 12 hours and further incubated with chemical inhibitors for 30 minutes as indicated. ON01910, BI2536 and CBB2001 are PLK1 inhibitors, VX680 and Aurora kinase inhibitor I are AURKA inhibitors, ZM447439 is a pan Aurora kinase inhibitor. Roscovitine is a pan CDKs inhibitor. (B) siRNA knockdown of AURKA abolished the mitotic modification of SOX2. PA-1 cells were transfected with siRNAs specific to Luciferase (Luc, as negative control) and AURKA for 48 hours, and then incubated with DMSO or nocodazole (Noc.) for 12 hours. Both short and long exposure results were showed. (C) AURKA interacts with SOX2 directly in M phase. PA-1 cells were treated with nocodazole (Noc.) or DMSO for 12 hours, and the protein complexes in the lysates were co-immunoprecipitated (co-IP) with antibodies.