Figure 2.

Activation of Rac1 by Tiam1 but not P-Rex1 increases E-cadherin levels at cell-cell contacts in A431 cells. A431 cells transduced with the doxycycline (dox)-inducible GEF expression system were treated with ethanol (- dox) or 1 μg ml ⁻¹ dox (+ dox) for 24 hours. Cells were then fixed and localization of E-cadherin was detected by immunofluorescence. Fluorescence markers against HA-tagged Tiam1 WT/GEF* or Myc-tagged P-Rex1 WT/GEF* were used to detect the expression of the respective GEF constructs upon dox induction. DAPI was used to visualize the nuclei. Scale bar = 20 µM.