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Experimental setup for pull-downs. (A) Amino acid sequence of SLBP fragment, sufficient to mediate S/G2 degration of SLBP. All the possible phosphoacceptor sites other than Thr 60 and Thr 61 were changed into alanine and were shown in light color. Mutations for the S/G2 stable version were shown below. (B) Schematic illustration of baits and experimental setup. (C) Amino acid sequence of DCAF11 with the peptides recovered in LC-MS/MS.
