Figure 2.
DCAF11 and Cul4A were pulled down by GST-SLBP fragment depending on phosphorylations on Thr 60 and Thr 61. HeLa cells were transfected with HA-DCAF11 (A) or Myc-Cul4A (B) and collected 48 hrs after transfection. Cells were lysed and pull-downs were performed with the indicated baits as explained in the materials and methods. GST-SLBPF (pTpTP) indicates phosphorylations on Thr 60 and Thr 61. Pull-downs were run on SDS-PAGE and immunoblotted for HA-DCAF11 or Myc-Cul4A. The amounts of baits were assessed by Comassie Briliant Blue (CBB) staining.