Figure 4.

DCAF11 interacts with wild type, but not Thr 61/Ala mutant SLBP. (A) HeLa cells were transfected with HA-DCAF11 along with hisSLBP. Cells were treated with MG132 for the last 4 hrs before collection. Cells were lysed and immunoprecipitations with nonspecific IgG or anti-SLBP were performed. Whole cell extract (input) and immunoprecipitates were analyzed by western blot for indicated proteins. (B) HeLa cells were transfected with HA-DCAF11 along with wild type hisSLBP (TTP) or Thr61/Ala mutant hisSLBP (TAP). Whole cell extracts (input) were subjected to immunoprecipitation (IP) with either nonspesific IgG or anti-HA and immunoprecipitates were immunoblotted for indicated proteins.