Figure 4. The influence of IL-1β retinal explants co-cultures.
(A) Quantitative RT-PCR of RT-PCR of IL-1β and IL-18 normalized with S26 mRNA in fresh human monocytes (hMo), hMo cultured alone or with a retinal explant for 18 hr (both on polycarbonate filters, n = 5, ANOVA, Dunnett’s post test*p=0,0079). (B and C) Oblique and perpendicular (insets) 3D reconstruction views of 18 hr peanut agglutinin (PNA)-stained retinal explant (B) and IL-1β (50 ng/ml) exposed explant (C). (D) Quantification of cone segment volume in retinal explants cultured with or without IL-1β (n = 6/group, Mann Whitney *p=0,0087). (E and F) Oblique and perpendicular (insets) 3D reconstruction views of 18 hr peanut agglutinin (PNA)-stained retinal explant co-cultured with human monocytes without (E) or with IL-1 receptor antagonist (F, 10 mg/ml). (G) Quantification of cone segment volume in Mo/retinal co-cultures with or without an IL-1 receptor antagonist (n = 6/group, Kruskal-Wallis, Dunn’s post test *p=0,0022 versus 'without hMo'; †p=0,0182 versus “with hMo without IL1-Ra). RetEx: retinal explant; CTL: control; CS: cone segment; hMo: human Monocytes. Scale bar B, C, E, F = 10 μm.