Skip to main content
. 2016 Aug;138(2):619–622.e7. doi: 10.1016/j.jaci.2016.03.007

Table E4.

Primers for amplification of cDNA

Region Orientation Sequence PCR cycles
EXON1-4 (279bp) FOR CGAAAATGCTTGACGCTGGC 94°C 3 min, 94°C 30 s, 54.6°C 30 s, 72°C 1 min × 40 cycles, 72°C 10 min
REV CTCGTCCTCGTCTGCAAAGT
EXON7-10 (303bp) FOR ACCCAGTGGATTCAAGCATGT 94°C 3 min, 94°C 30 s, 54.6°C 30 s, 72°C 1 min × 40 cycles, 72°C 10 min
REV GGACCAGAACGACCCTTGTT
EXON2-7 (449bp) FOR GTCCTACTTCATCCGCCTTTAC 94°C 3 min, 94°C 30 s, 55.8°C 30 s, 72°C 1 min × 40 cycles, 72°C 10 min
REV TCTTCCCTGAGCGTTTCTTATC
EXON7-8 (73bp) FOR GTGGATTCAAGCATGTCAGCC 94°C 3 min, 94°C 30 s, 55.8°C 30 s, 72°C 1 min × 40 cycles, 72°C 10 min
REV TCTGGGTCGAGGTTGTTCAC