Table E4.
Primers for amplification of cDNA
| Region | Orientation | Sequence | PCR cycles |
|---|---|---|---|
| EXON1-4 (279bp) | FOR | CGAAAATGCTTGACGCTGGC | 94°C 3 min, 94°C 30 s, 54.6°C 30 s, 72°C 1 min × 40 cycles, 72°C 10 min |
| REV | CTCGTCCTCGTCTGCAAAGT | ||
| EXON7-10 (303bp) | FOR | ACCCAGTGGATTCAAGCATGT | 94°C 3 min, 94°C 30 s, 54.6°C 30 s, 72°C 1 min × 40 cycles, 72°C 10 min |
| REV | GGACCAGAACGACCCTTGTT | ||
| EXON2-7 (449bp) | FOR | GTCCTACTTCATCCGCCTTTAC | 94°C 3 min, 94°C 30 s, 55.8°C 30 s, 72°C 1 min × 40 cycles, 72°C 10 min |
| REV | TCTTCCCTGAGCGTTTCTTATC | ||
| EXON7-8 (73bp) | FOR | GTGGATTCAAGCATGTCAGCC | 94°C 3 min, 94°C 30 s, 55.8°C 30 s, 72°C 1 min × 40 cycles, 72°C 10 min |
| REV | TCTGGGTCGAGGTTGTTCAC |