EXON1-4 (279bp) |
FOR |
CGAAAATGCTTGACGCTGGC |
94°C 3 min, 94°C 30 s, 54.6°C 30 s, 72°C 1 min × 40 cycles, 72°C 10 min |
|
REV |
CTCGTCCTCGTCTGCAAAGT |
|
EXON7-10 (303bp) |
FOR |
ACCCAGTGGATTCAAGCATGT |
94°C 3 min, 94°C 30 s, 54.6°C 30 s, 72°C 1 min × 40 cycles, 72°C 10 min |
|
REV |
GGACCAGAACGACCCTTGTT |
|
EXON2-7 (449bp) |
FOR |
GTCCTACTTCATCCGCCTTTAC |
94°C 3 min, 94°C 30 s, 55.8°C 30 s, 72°C 1 min × 40 cycles, 72°C 10 min |
|
REV |
TCTTCCCTGAGCGTTTCTTATC |
|
EXON7-8 (73bp) |
FOR |
GTGGATTCAAGCATGTCAGCC |
94°C 3 min, 94°C 30 s, 55.8°C 30 s, 72°C 1 min × 40 cycles, 72°C 10 min |
|
REV |
TCTGGGTCGAGGTTGTTCAC |
|