Figure 3. Statistics of nanomechanical probing based on single–tap measurements.

(a) Stiffness probing uncertainty is the lowest median coefficients of variance of all pixels from an image and its scrambled control, i.e., ΔCV₅₀ = CV_50,original − CV_50,scrambled. (b) Zoomed–in region of the stiffness map (from Figure 2d) to show E*_load value from each single tap. Tap–to–tap spacing was ~0.3 nm. (c) Histograms of the spectrin and surrounding membrane regions based on the single–tap stiffness in (b). The inset image shows the selected spectrin regions marked in blue.