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. Author manuscript; available in PMC: 2017 Aug 15.
Published in final edited form as: Neurosci Lett. 2016 Jun 21;628:213–218. doi: 10.1016/j.neulet.2016.06.042

Figure 3. Cell surface expression of CCCs in CA1 and DG of chronically epileptic animals.

Figure 3

Following labeling, microdissection and batch extraction of biotinylated proteins, samples from the CA1 and DG of hippocampus obtained from chronically epileptic animals with either recent/frequent or infrequent seizures were analyzed by Western blot. (A and D) Representative blots for KCC2, NKCC1 and actin. Densitometry analysis of (B) KCC2 or (C) NKCC1, immunoreactivity in CA1 samples from epileptic animals normalized to actin is presented as the mean ± SEM of five independent experiments. Densitometry analysis of (E) KCC2 or (F) NKCC1 immunoreactivity in DG of chronically epileptic animals normalized to actin presented as the mean ± SEM of five independent experiments. The signal detected at the epileptic animals was compared to controls by ANOVA followed by Bonferroni post hoc test (**p < 0.01, ***p < 0.001).