Fig. 2. SERT^GluΔ but not SERT^CortexΔ or SERT^RapheΔ abolishes SERT expression in primary sensory cortices.

A. SERT (red) and Vglut2 (green) double immunostaining of tangential sections of primary visual (v), auditory (a) and somatosensory barrel (s) cortices in P7 mutant and control SERT^fl/fl littermate mice. SERT staining in Vglut2+ patches in SERT^CortexΔ mice was indistinguishable from the SERT^fl/fl control or SERT^RapheΔ mice, whereas the SERT staining in SERT^GluΔ mice was diminished as in SERT^Null mice. B. SERT and 5-HT immunostaining (both grayscale) of adjacent coronal sections of raphe nuclei from P7 mice. SERT staining was abolished in SERT^Null, diminished in SERT^RapheΔ, but unaffected in SERT^GluΔ and SERT^CortexΔ mice. Aq, aqueduct. Some images for SERT^Null, SERT^RapheΔ and SERT^GluΔ brain sections are reproduced from a previous study (Chen et al., 2015) for comparison of SERT immunostaining patterns in SERT^CortexΔ brain sections. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)