FIG 1.
Genetic comparison and expression of EIV and CIV PA-X in cell culture. (A) Comparison of EIV and CIV PA-X consensus protein sequences revealed three differences, two point mutations and one deletion. (B) Expression of PA-X was detected after IVT by Western blotting using a rabbit anti-PA-X peptide antibody. (C) EIV and CIV PA-X gene suppression ability was assayed by β-galactosidase reporter assay in a dose-dependent manner. Error bars represented the standard deviation of three independent experiments.
