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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Virology. 2016 Jun 20;496:186–193. doi: 10.1016/j.virol.2016.06.006

Figure 2. Expression of WNV proteins and VLP release in transfected cells.

Figure 2

Vero cells mock-transfected or transfected with the plasmids expressing the indicated WNV construct were harvested at 72 hours post-transfection. An equal volume (4% of total sample) of either cell lysate or clarified supernatant was resolved by SDS-PAGE, transferred to a membrane, and the membrane was probed with an anti-E monoclonal antibody. The position of the 48.8 kDa ladder band is indicated. (A) Comparison of preprotrypsin leader (lane 2), leaderless (lane 3), and native E (lane 4) expressing constructs in transfected Vero cells. (B) Comparison of the VLP production by the indicated constructs as measured by E in the cells and supernatant.