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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Virology. 2016 Jun 16;496:175–185. doi: 10.1016/j.virol.2016.06.004

Figure 1. STAT3 is phosphorylated on conserved tyrosine residue 705 in various cell types following RVFV infection.

Figure 1

A) Vero, HSAEC, or MEF cells were mock-infected or infected with MP12 or MP12-ΔNSs at MOI 3.0 for one hour. Cell lysates were collected at 8, 16, and 24 hpi and analyzed by western blot. Membranes were probed for p-STAT3 (Y705), RVFV nucleoprotein (NP), and β-actin as a loading control. B) Vero cells were transfected with 2.5 μg of NSs-Flag plasmid or pcDNA as a control. At 24 hours post transfection, cell lysates were collected and analyzed by western blot for p-STAT3 (Y705), RVFV NSs (Flag-tag), and β-actin as a loading control. Blots from two independent experiments (5 biological replicates) were quantified for p-STAT3 (Y705) and a representative blot is shown. ** p ≤ 0.005