FIGURE 1.

Cytotoxicity of Ochratoxin A (OTA) in Neuro-2a cells and its reversal by N-acetylcysteine (NAC). (A) Neuro-2a cells were treated without or with various concentrations of OTA (50–750 nM) for 24 h. Cells survival was measured by MTT assay. (B) Neuro-2a cells were pretreated without or with NAC (0–1 mM) for 1 h and challenged with different concentrations of OTA for 24 h and viability was accessed by MTT assay. (C) Membrane damage was measured by LDH leakage assay. (D) Neuro-2a cells were pretreated without or with NAC (1 mM) for 1 h and challenged with OTA (500 nM) for 24 h and viability was accessed by LDH leakage assay. The data represented as mean ± SEM of three independent experiments. ^∗p < 0.05 versus respective control group and ^#p < 0.05 versus respective OTA treated group.