FIGURE 2.

Effect of OTA on reactive oxygen species (ROS) production in Neuro-2a and its scavenging by NAC. (A) Neuro-2a cells were treated with various concentrations of OTA (100–500 nM) for 24 h. ROS levels were monitored by using DCFH-DA (B). Neuro-2a cells were pretreated with NAC (1 mM) for 24 h followed by OTA (500 nM) treatment for 24 h and intracellular ROS was quantified by using DCFH-DA. The data represented as mean ± SEM of three independent experiments. ^∗p < 0.05 versus respective control group and ^#p < 0.05 versus respective OTA treated group.