FIGURE 5.

NAC reversed the mitochondrial membrane potential (MMP) loss induced by OTA treatment in Neuro-2a cells. (A) Neuro-2a cells were treated without or with various concentrations of OTA (100–500 nM) for 24 h and MMP loss was quantified as fluorescence (B). Neuro-2a cells were pretreated with NAC (1 mM) for 24 h followed by OTA (500 nM) treatment for 24 h and MMP loss was quantified by using rhodamine123. The data represented as mean ± SEM of three independent experiments. ^∗p < 0.05 versus respective control group and ^#p < 0.05 versus respective OTA treated group.