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. 2016 Aug 2;7:1107. doi: 10.3389/fpls.2016.01107

Figure 1.

Figure 1

Assessment of OG treatment efficacy and plasma membrane protein enrichment. (A) Detection of the phosphorylation of Arabidopsis thaliana MAPKs MPK6 and MPK3 upon treatment of seedlings for 10 min with OGs by immunoblot analysis using an anti-p44/42-ERK antibody (top panel). Thirty micrograms of each protein sample were loaded. Levels of MPK3 and MPK6 total proteins were determined using specific antibodies (bottom panel). The identity of individual MAP kinases, as determined by size, is indicated by arrows. (B) Western blot analysis of total protein extracts, TMF proteins directly solubilized in Laemmli buffer (TMF) or immediately before DIGE analysis (DIGE-TMF), probed with antibody against the plasma membrane H+-ATPase. Four micrograms of each protein sample were loaded. (C) The analysis of enriched GO terms for the cellular component category within the TMF proteins identified (listed in Data Sheet S2A in Supplementary Material) using the AgriGO database. Barplot shows selected GO terms significantly enriched (FDR < 0.05, Yekutieli adjusted). The background/reference represents the proportion of all annotated genes of each GO term within the total genes in the TAIR10 database.