Table 1.
List of LC-MS/MS identified phosphosites regulated by OGsa.
| Protein nameb | TAIR IDc | Locd | Uniprot IDe | Posf | Phosphopeptide (best evidence)g | Average log2 ratio Tr/Ch | Log2 ratio Tr1/C1i | Log2 ratio Tr2/C2i | μarray OG 1 h/3 hj |
|---|---|---|---|---|---|---|---|---|---|
| TRANSPORT | |||||||||
| Aquaporin PIP2-1; Aquaporin PIP2-2; Aquaporin PIP2-3 |
At3g53420; At2g37170; At2g37180 |
PM§ | P43286; P43287; P30302 | 280; 278 278 |
SI GS(0.996)FRS(0.004)AANV | Tr | Tr | Tr | ns |
| ABC transporter B family member 20 (ABC-B20) | At3g55320 | PM | Q9M3B9 | 712 | GS(0.001)GVFRPQEICFDT(0.978)EES(0.054)PKAHS(0.216)PAS(0.751)EK | Tr | Tr | Tr | ns |
| Translocase of chloroplast 159 (TOC159) | At4g02510 | PL§ | O81283 | 686 | IDGQIVT(0.003)DS(0.996)DEDVDT(0.001)EDEGEEK | Tr | Tr | Tr | ns |
| ABC transporter C family member 14 (ABCC14) | At3g62700 | VO | Q9LZJ5 | 903 | S(0.348)IS(0.077)IES(0.575)PRQPKS(1)PK | Tr | Tr | Tr | −1.5/ns |
| ABC transporter C family member 14 (ABCC14) | At3g62700 | VO | Q9LZJ5 | 925 | T(0.333)T(0.333)S(0.333)MES(0.999)PRISEINDESIK | Tr | Tr | Tr | −1.5/ns |
ABC transporter G family member 36 (PEN3) 
|
At1g59870 | PM§ | Q9XIE2 | 45 | T(0.011)QS(0.989)VNDDEEAIK | 5.70 | 4.77 | 6.62 | 2.8/ns |
| ABC transporter C family member 14 (ABCC14) | At3g62700 | VO | Q9LZJ5 | 897 | S(0.009)IS(0.991)IES(1)PRQPKS(1)PK | 4.63 | 3.24 | 6.02 | −1.5/ns |
| ABC transporter C family member 14 (ABCC14) | At3g62700 | VO | Q9LZJ5 | 894 | S(0.009)IS(0.991)IES(1)PRQPKS(1)PK | 4.55 | 3.24 | 5.87 | −1.5/ns |
| ATPase 1 (AHA1), plasma membrane
|
At2g18960 | PM§ | P20649 | 544 | DS(1)NIASIPVEEIIEK | 4.17 | 3.83 | 4.50 | ns |
| Transport protein particle (TRAPP) component | At3g05000 | CY | Q9CAW4 | 121 | VSIDPSSENETQDPST(0.999)PGES(0.001)K | 3.98 | 4.17 | 3.79 | ns |
| ATPase 2 (AHA2), plasma membrane; ATPase 1 (AHA1), plasma membrane
|
At4g30190; At2g18960 |
PM§ |
P19456; P20649 |
899; 899 |
GS(1)YREISEIAEQAK | 3.42 | 3.08 | 3.77 | ns/2.3 |
| Copper transporter 5 (CuTR5) | At5g20650 | GO§ | Q93VM8 | 68 | S(0.018)S(0.018)S(0.962)GVS(0.001)APIIPK | C | C | C | ns |
| UDP-galactose transporter 1 | At1g77610 | GO | Q9C521 | 310 | HMIS(0.001)QQT(0.006)PGT(0.821)PRT(0.821)PRT(0.35)PR | C | C | C | −1.6/ns |
| UDP-galactose transporter 1 | At1g77610 | GO | Q9C521 | 313 | HMIS(0.001)QQT(0.006)PGT(0.821)PRT(0.821)PRT(0.35)PR | C | C | C | −1.6/ns |
| NSP (Nuclear shuttle protein)-interacting GTPase | At4g13350 | CY | Q8W4K6 | 407 | S(0.214)MS(0.779)APS(0.007)IQPIQGVPSGGIQSSEVKPSGR | C | C | C | na |
| NSP (Nuclear shuttle protein)-interacting GTPase | At4g13350 | CY | Q8W4K6 | 219 | T(0.045)S(0.045)EEGS(0.185)QS(0.725)PEQVKDIGS(0.199)AS(0.801)PPVARPVR | C | C | C | na |
| SIGNAL TRANSDUCTION | |||||||||
| Transcription regulator NOT2/NOT3/NOT5 family protein | At5g18230 | NU | F4JWJ6 | 431 | NIMGVESNVQPIT(0.043)S(0.891)PIS(0.066)K | Tr | Tr | Tr | ns |
| Transducin family protein (WD-40 RFP) | At5g05570 | MI§ | F4K0R0 | 464 | ART(0.928)PRT(0.661)PS(0.303)GES(0.107)AQWPIT(0.002)GGVPSHVDDYK | Tr | Tr | Tr | ns |
| Phospholipase like protein (PEARLI 4f) family | At4g38550 | NU | Q9C5F6 | 232 | EQFEDIYEQDGDVT(1)PR | Tr | Tr | Tr | 6.7/ns |
| Phototropin-2 (PHOT2) | At5g58140 | PM§ | F4KDJ3 | 504 | GEIQYFIGVQIDGSDHVEPIQNRIS(1)ER | Tr | Tr | Tr | ns |
| Mitogen-activated protein kinase 6 (MPK6) | At2g43790 | CY§ | Q39026 | 221 | VTSESDFMT(1)EY(1)VVTR | Tr | Tr | Tr | ns |
| Mitogen-activated protein kinase 6 (MPK6) | At2g43790 | CY§ | Q39026 | 223 | VTSESDFMT(1)EY(1)VVTR | Tr | Tr | Tr | ns |
| Calmodulin-binding protein (EDA39) | At4g33050 | CY |
O82645; F4JVX1 |
462; 500 |
VS(0.001)PANS(0.003)Y(0.003)GPIPS(0.996)PRPS(0.998)PK | Tr | Tr | Tr | 24.1/2.9 |
| Calmodulin-binding protein (EDA39) | At4g33050 | CY |
O82645; F4JVX1 |
466; 504 |
VS(0.001)PANS(0.003)Y(0.003)GPIPS(0.996)PRPS(0.998)PK | Tr | Tr | Tr | 24.1/2.9 |
| Phospholipase D gamma 1(PLDγ1) | At4g11850 | VO | Q9T053 | 680 | EVPVGTVS(0.001)VY(0.014)NS(0.984)PR | 3.83 | 4.47 | 3.20 | ns |
| Protein kinase family protein | At5g03320 | PM | Q9LZF8 | 17 | DEQRS(1)PKPVS(0.806)PT(0.082)S(0.027)NFS(0.085)DVNK | 2.79 | 3.15 | 2.43 | 2.5/ns |
| Putative tyrosine phosphatase (PTEN2a) | At3g19420 | NU | Q9LT75 | 509 | ETENPDKDDVFS(1)DNEGDSTGPTK | 2.62 | 2.42 | 2.81 | 1.5/ns |
| Cyclin-dependent protein kinase-like protein | At5g44290 | NU | Q9FKV9 | 75 | HQEIAEIGDT(1)DEDEDDDHHPPEEIK | −2.67 | −2.31 | −3.03 | 2.9/ns |
| Auxin response factor ARF1 | At1g59750 | NU | F4ID31 | 399 | PRPPGIPS(0.084)PAT(0.06)GPS(0.163)DGVWKS(0.159)PADT(0.664)PS(0.825)S(0.825)VPIFS(0.221)PPAK | −2.74 | −2.98 | −2.50 | ns |
| Auxin response factor ARF1 | At1g59750 | NU | F4ID31 | 400 | PRPPGIPS(0.084)PAT(0.06)GPS(0.163)DGVWKS(0.159)PADT(0.664)PS(0.825)S(0.825)VPIFS(0.221)PPAK | −2.74 | −2.98 | −2.50 | ns |
| Serine/threonine protein kinase | At1g48210 | PM |
F4HWU0; Q9LUT0 |
353; 354 |
AIQPIINPPRS(0.986)APQT(0.986)PHRNPY(0.027) | C | C | C | ns |
| Serine/threonine protein kinase | At1g48210 | PM |
F4HWU0; Q9LUT0 |
357; 358 |
AIQPIINPPRS(0.986)APQT(0.986)PHRNPY(0.027) | C | C | C | ns |
| MAP3K epsilon protein kinase (MKKK7) | At3g13530 | PM§ | Q9LJD8 | 788 | VRS(1)GQIDPNNPIFGQNETSSISMIDQPDVIK | C | C | C | ns |
| Probable LRR receptor-like serine/threonine-protein kinase | At5g37450 | PM | C0LGU1 | 268 | S(0.886)IVIY(0.059)Y(0.059)IDIS(0.578)S(0.889)NKIT(0.528)GEIPKNK | C | C | C | ns |
| Probable LRR receptor-like serine/threonine-protein kinase | At5g37450 | PM | C0LGU1 | 278 | S(0.886)IVIY(0.059)Y(0.059)IDIS(0.578)S(0.889)NKIT(0.528)GEIPKNK | C | C | C | ns |
| GSK3/Shaggy-related protein kinase alpha (ASK1) | At5g26751 | PM | P43288 | 229 | GEPNIS(0.232)Y(0.768)ICSR | C | C | C | 1.6/ns |
| Probable serine/threonine-protein kinase | At1g18390 | PM | P0C5E2 | 643 | S(0.003)GPIVAQS(0.761)PDS(0.235)VIVK | C | C | C | 6.8/1.5 |
| Phospholipase-like protein (PEARLI 4)
|
At2g20960 | NU | Q9SKR5 | 314 | S(0.829)KT(0.169)PEPQPT(0.001)Y(0.001)FEPSSR | C | C | C | 4.0/ns |
| TRAFFICKING | |||||||||
| Patellin-2 (PATL2) | At1g22530 | PM | Q56ZI2 | 79 | EIIQS(0.064)ES(0.936)FK | Tr | Tr | Tr | −2.0/−1.6 |
| Patellin-4 (PATL4) | At1g30690 | PM | Q94C59 | 53 | S(0.126)AS(0.874)FKEESDFFADIK | Tr | Tr | Tr | ns |
| ADP-ribosylation factor GTPase-activating protein (ZIGA4) | At1g08680 | NU | F4HXP0 | 236 | S(0.001)DIQS(0.999)PNFQQEAEFR | Tr | Tr | Tr | ns |
| Kinesin-like protein KAC1 | At5g10470 | CY§ | Q9LX99 | 611 | TGDAIQS(0.001)QDIFS(0.999)PIPK | Tr | Tr | Tr | ns |
| Kinesin-like protein KAC1 | At5g10470 | CY§ | Q9LX99 | 698 | GEGYSAEAVAIPS(0.063)T(0.937)PNK | Tr | Tr | Tr | ns |
| 65-kDa microtubule-associated protein 1 (MAP65-1) | At5g55230 | NU; CS§ | F4K3E4; Q9FLP0 | 615; 586 |
EEAASSPVSGAADHQVPAS(1)P | Tr | Tr | Tr | ns |
| Myosin-17 (XIK)
|
At5g20490 | ER§ | F4K5J2 | 990 | QQAIAIS(0.957)PT(0.041)S(0.002)R | 3.86 | 5.22 | 2.49 | ns |
| Patellin-3 (PATL3) | At1g72160 | CY | Q56Z59 | 108 | SMIPQNIGS(1)FK | 3.30 | 2.95 | 3.66 | ns |
| Membrane fusion protein Use1 | At3g55600 | NU | Q6NKR3 | 104 | IEDEPRS(0.999)PT(0.973)S(0.028)PQIR | 2.42 | 2.91 | 1.94 | 1.8/ns |
| Syntaxin of plants 121 (SYP121)
|
At3g11820 | PM§; GO§ |
Q9ZSD4 | 264 | AS(0.213)S(0.755)FIRGGT(0.031)DQIQTAR | −3.06 | −2.37 | −3.74 | 7.5/ns |
| Syntaxin of plants 122 (SYP122)
|
At3g52400 | PM§ | Q9SVC2 | 18 | MIAIFHEAFAHPPEEINS(0.983)PAS(0.017)EK | −4.08 | −2.61 | −5.54 | 11.5/ns |
| RESPONSE TO STRESS | |||||||||
| Respiratory burst oxidase homolog protein D | At5g47910 | PM§ | Q9FIJ0 | 343 | IIS(1)QMIS(1)QK | Tr | Tr | Tr | 9.9/ns |
| Respiratory burst oxidase homolog protein D | At5g47910 | PM§ | Q9FIJ0 | 347 | IIS(1)QMIS(1)QK | Tr | Tr | Tr | 9.9/ns |
| Metal tolerance protein C2 | At3g12100 | VO | F4J8M5 | 37 | S(0.99)FQQS(0.212)HGPRT(0.688)PAVT(0.11)EAAKPFIDR | Tr | Tr | Tr | ns |
| Aluminium induced protein with YGL and LRDR motifs | At5g43830 | CY | Q9FG81 | 18 | TVANSPEAIQS(0.997)PHS(0.003)SESAFAIK | 5.95 | 6.83 | 5.06 | ns |
| Aluminium induced protein with YGL and LRDR motifs | At4g27450 | CY | Q93V62 | 219 | VDS(1)EGVICGANFK | −2.83 | −3.56 | −2.10 | ns |
| Protein plant cadmium resistance 8 (CdRes8) | At1g52200 | PM | Q9M815 | 12 | GRVT(0.002)T(0.002)PS(0.184)EEDS(0.812)NNGIPVQQPGT(1)PNQR | C | C | C | 6.1/2.3 |
| Putative defensin-like protein 135 | At4g09647 | EX | Q2V3K1 | 2 | S(0.946)KT(0.849)FQPS(0.176)IVMIT(0.036)IFIIIVT(0.996)S(0.997)QR | C | C | C | ns |
| Putative defensin-like protein 135 | At4g09647 | EX | Q2V3K1 | 21 | S(0.946)KT(0.849)FQPS(0.176)IVMIT(0.036)IFIIIVT(0.996)S(0.997)QR | C | C | C | ns |
| Putative defensin-like protein 135 | At4g09647 | EX | Q2V3K1 | 4 | S(0.946)KT(0.849)FQPS(0.176)IVMIT(0.036)IFIIIVT(0.996)S(0.997)QR | C | C | C | ns |
| Putative defensin-like protein 135 | At4g09647 | EX | Q2V3K1 | 20 | S(0.946)KT(0.849)FQPS(0.176)IVMIT(0.036)IFIIIVT(0.996)S(0.997)QR | C | C | C | ns |
| Heat shock protein 90-1 (HSP90-1) | At5g52640 | CY; NU |
P27323 | 219 | EIS(1)DDEDEDEPK | C | C | C | 5.0/4.6 |
| PROTEIN METABOLIC PROCESS | |||||||||
| Pumilio homolog 3; P1umilio homolog 1; Pumilio homolog 2 |
At2g29140; At2g29200; At2g29190 |
CY; NU |
Q9ZW02; Q9ZW07; Q9ZW06 |
268; 272; 280 |
VPS(1)PCIT(0.999)PIGS(0.001)GR | Tr | Tr | Tr | ns |
| Pumilio homolog 3; Pumilio homolog 1; Pumilio homolog 2 |
At2g29140; At2g29200; At2g29190 |
CY; NU |
Q9ZW02; Q9ZW07; Q9ZW06 |
272; 276 284 |
VPS(1)PCIT(0.999)PIGS(0.001)GR | Tr | Tr | Tr | ns |
| 40S ribosomal protein S3-2 | At3g53870 | CY | Q9M339 | 212 | TPIPDVVIIHS(1)PK | Tr | Tr | Tr | ns |
| RING/U-box domain-containing protein | At3g06330 | GO | B3H497 | 144 | IS(0.989)PVVS(0.004)T(0.004)QIS(0.002)AGVPGDPPNK | Tr | Tr | Tr | ns |
| Enhancer of mRNA-decapping protein 4 | At3g13300 | CY | Q9LTT8 | 98 | TISYPTPPINIQS(1)PR | 4.28 | 4.09 | 4.47 | ns |
| Eukaryotic translation initiation factor 4G (EIF4G) | At3g60240 | CY | A8MR97; Q76E23 | 1525 1529 | QVIQGPSAT(0.005)VNS(0.995)PR | 3.45 | 3.37 | 3.53 | ns |
| 40S ribosomal proteinS2-3 | At2g41840 | CY | P49688 | 273 | AIS(0.845)T(0.149)S(0.006)KPDPVVEDQA | 2.71 | 2.51 | 2.91 | ns |
| Serine/arginine-rich splicing factor 30 (SCL30) | At3g55460 | NU§ | Q8L3X8 | 204 | RS(1)Y(0.004)S(0.996)PGYEGAAAAAPDRDR | 1.84 | 1.26 | 2.43 | ns |
| Serine/arginine-rich splicing factor 30 (SCL30) | At3g55460 | NU§ | Q8L3X8 | 206 | RS(1)Y(0.004)S(0.996)PGYEGAAAAAPDRDR | 1.84 | 1.26 | 2.43 | ns |
| Putative translation initiation factor eIF-2B epsilon subunit (eIF-2B) | At2g34970 | CY | O64760 | 447 | VSIIQQPT(0.049)T(0.049)EDS(0.899)DEEIEY(0.003)ADSSSGTADHISGINIQMESK | C | C | C | ns |
| METABOLISM | |||||||||
| NADPH-cytochrome P450 reductase (CPR)
|
At4g24520 | ER | F4JQY4 | 248 | S(0.002)VAT(0.993)PYT(0.005)AVIPEYR | Tr | Tr | Tr | ns |
| Glutamate decarboxylase 1 | At5g17330 | CY; PM; GO | Q42521 | 8 | VIS(0.064)HAVS(0.751)ES(0.232)DVS(0.935)VHS(0.008)T(0.007)FAS(0.003)R | Tr | Tr | Tr | ns |
| 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (FKFBP) | At1g07110 | ER§; GO§ |
Q9MB58 | 276 | SVETIS(1)PFQQK | 2.39 | 2.23 | 2.55 | ns |
| Photosystem Q(B) protein (psbA) | AtCg00020 | PL | P83755 | 2 | T(1)AIIERR | −2.57 | −2.42 | −2.71 | na |
| Probable pectin methyltransferase PMT13 (QUA3) | At4g00740 | GO | Q93W95 | 280 | CIIPFT(0.775)AY(0.224)NAT(0.252)Y(0.749)FIEVDRIIR | −2.93 | −2.04 | −3.82 | −2.0/ns |
| Probable pectin methyltransferase PMT13 (QUA3) | At4g00740 | GO | Q93W95 | 286 | CIIPFT(0.53)AY(0.468)NAT(0.037)Y(0.966)FIEVDRIIR | −2.93 | −2.04 | −3.82 | −2.0/ns |
| Cytochrome P450, family 77, subfamily A, polypeptide 6 (CYP77A6) | At3g10570 | ER | Q9SQY7 | 284 | AIQKPGT(0.927)DKT(0.164)AS(0.154)S(0.774)FS(0.754)Y(0.262)IDT(0.964)IFDIK | −4.38 | −4.92 | −3.84 | ns |
| Cytochrome P450, family 77, subfamily A, polypeptide 6 (CYP77A6) | At3g10570 | ER | Q9SQY7 | 282 | AIQKPGT(0.927)DKT(0.164)AS(0.154)S(0.774)FS(0.754)Y(0.262)IDT(0.964)IFDIK | −4.50 | −4.92 | −4.08 | ns |
| ADP-glucose pyrophosphorylase family protein | At1g74910 | CY | F4HXD1 | 217 | RVS(0.763)S(0.237)FEAIQPATR | C | C | C | ns |
| OTHER PROTEINS | |||||||||
| Tetratricopeptide repeat domain protein (TSS) | At4g28080 | PX | F4JKH6 | 1311 | TSSNEISISVAGS(0.003)T(0.044)S(0.181)S(0.759)PAS(0.014)K | Tr | Tr | Tr | ns/−1.7 |
| DEAD-box ATP-dependent RNA helicase 38 (LOS-4) | At3g53110 | CY; NU |
Q93ZG7 | 25 | ADTVEKVPTVVES(0.005)S(0.004)S(0.007)S(0.013)S(0.011)T(0.011)VEAS(0.186)NS(0.762)AEK | Tr | Tr | Tr | ns |
| Putative bZIP protein | At3g60320 | NU | Q93YU8 | 147 | IPHIIS(0.15)ES(0.649)S(0.189)PS(0.019)S(0.08)S(0.912)PR | Tr | Tr | Tr | −3.0/ns |
| Nuclear/nucleolar GTPase (AtNug2) | At1g52980 | NU | Q9C923 | 116 | ERKIPMS(1)IIT(1)DNK | Tr | Tr | Tr | ns |
| Nuclear/nucleolar GTPase (AtNug2) | At1g52980 | NU | Q9C923 | 119 | ERKIPMS(1)IIT(1)DNK | Tr | Tr | Tr | ns |
| Uncharacterized protein | At1g77765 | CY | Q6DYD4 | 94 | CDVVES(0.014)DNKPERIS(0.926)PS(0.059)PK | Tr | Tr | Tr | na |
| Uncharacterized protein | At1g19530 | NU | Q93WK6 | 23 | S(0.865)IT(0.135)REEIDTFWK | Tr | Tr | Tr | ns |
| Uncharacterized membrane protein | At3g27390 | PM | Q8GUM4 | 544 | DES(0.953)IT(0.047)EPPAPVK | Tr | Tr | Tr | ns |
| Uncharacterized protein | At5g13260 | NU | Q8VZL1 | 446 | ISDIEIKS(1)PGGPK | 3.65 | 3.11 | 4.20 | ns |
| SAP domain-containing protein
|
At4g39680 | NU | O65655 | 417 | VPEAQIT(0.002)NS(0.003)AT(0.995)PT(0.017)T(0.053)T(0.931)PR | 2.91 | 3.20 | 2.62 | ns |
| SAP domain-containing protein
|
At4g39680 | NU | O65655 | 421 | VPEAQIT(0.44)NS(0.28)AT(0.28)PTT(0.001)T(0.999)PR | 2.57 | 2.53 | 2.62 | ns |
| Uncharacterized protein | At5g52980 | CY | Q9LVV4 | 10 | DQPDPKNGS(0.99)GIIIS(0.922)AT(0.088)EPIR | −1.99 | −1.63 | −2.35 | ns |
| Uncharacterized protein | At5g52980 | CY | Q9LVV4 | 15 | DQPDPKNGS(0.99)GIIIS(0.922)AT(0.088)EPIR | −1.99 | −1.63 | −2.35 | ns |
| Midasin (MDN1) | At1g67120 | NU | F4HRR8 | 4797 | CGS(1)PQKEEPGNDIEQEPETEPIEGK | C | C | C | ns |
| DEK domain-containing chromatin associated protein (DEK-C) | At4g26630 | CY | Q9SUA1 | 523 | SIAHS(0.004)DDES(0.996)EEEKEEEEK | C | C | C | ns |
| DNA topoisomerase 1 beta | At5g55310 | NU | Q9FJ79 | 126 | APS(0.002)VS(0.073)KS(0.9)DDEDS(0.024)EDDKPISAR | C | C | C | ns |
| BTB/POZ domain-containing protein | At5g67385 | PM | Q66GP0 | 558 | TSSSTIS(0.004)T(0.036)NPS(0.474)S(0.474)PIS(0.025)T(0.025)AS(0.068)T(0.895)GKPPIPR | C | C | C | na |
| Uncharacterized protein | At1g20970 | PM | F4HWC3 | 203 | NNVEEPEVEIES(0.065)DS(0.162)ET(0.773)DVEGHQGDK | C | C | C | ns |
| Uncharacterized protein
|
At2g15860 | NU |
Q8L4R9 ; F4IJD7 |
178; 190 |
IENS(0.005)VQQGS(0.101)S(0.894)PR | C | C | C | ns |
| Uncharacterized protein | At4g31880 | NU | F4JTF2; Q8GUP3 | 558; 559 |
AIDEES(0.002)IHT(0.053)S(0.88)S(0.064)GDNEKPAVSSGK | C | C | C | ns |
| Uncharacterized protein | At5g64090 | PL | Q9FMI8 | 8 | MDFS(0.034)VKPS(0.772)GGS(0.159)PS(0.034)PSSSTSSSTPHR | C | C | C | 1.6/ns |
a
Phosphosites in each functional category are listed according to the average log2 ratio (Treated/Control), in descending order.
b
Full name of the identified protein. Proteins that have never been described before as phosphorylated are underlined. indicates proteins that have been identified as regulated by OGs in Kohorn et al. (2016).
c
ID of the identified protein from the TAIR database (The Arabidopsis Information Resource database. www.arabidopsis.org)
d
Subcellular localization, obtained from SUBA (the SUBcellular localization database for Arabidopsis proteins, http://suba3.plantenergy.uwa.edu.au/). PL, plastid; EX, extracellular; CY, cytosol; PM, plasma membrane; ER, endoplasmic reticulum; GO, Golgi apparatus; VO, vacuole; NU, nucleus; PX, peroxisome; MI, mitochondrion; CS, cytoskeleton. §indicates localization determined by GFP fusion.
e
ID of the identified protein, from the UniProtKB database (www.uniprot.org/). Proteins that have never been described before as phosphorylated at the residues reported here are ud.
f
Position of the phosphorylated amino acid on the whole protein sequence.
g
Phosphopeptide sequence with the localization probability score assigned to each site and the phosphorylated amino acid (bold and ud).
h
Average fold change (log2) between corresponding OG-treated and control phosphosites (Tr/C). Positive and negative values indicate increases and decreases in phosphorylation, respectively, upon treatment. C indicates proteins that are exclusively present in control samples and that are not detected in all replicates of OG- treated samples. Tr indicates proteins that are exclusively present in OG-treated samples and that are not detected in all replicates of control samples.
i
Fold change (log2) between corresponding OG-treated and control phosphosites (Tr/C) in each replicate.
j
Fold change of transcript levels after OG treatment at 1 and 3 h with respect to the mock-treated control (Denoux et al., 2008). Only data of genes for which fold change is significant and ≥1.5 in at least one time-point are shown. ns, not significant (P > 0.01); na, not applicable.