Table 1.
BAC clone | Target gene | Verification by sequencing with target gene-based primers | Target gene sequence variant | FISH signal | NLL | Assigned sequence (BAC or scaffold) | Synteny (based on assigned sequence) |
---|---|---|---|---|---|---|---|
002B02 | Random | − | − | Single | 4 | KB439932.1 | + |
004L17 | NOD45 | + | 1 | Single | − | − | − |
008A17 | NOD45 | + | 1 | Single | − | 138H12 | + |
008O20 | ENOD40 | + | 1 | Single | 17 | 131P18 | + |
010I16 | ENOD40 | − | − | Repetitive | − | − | − |
018I03 | ENOD40 | + | 1 | Single | − | 131P18 | + |
020A06 | Random | NI | NI | Single | 11 | AOCW01152381.1, KB427843.1 | − |
024K15 | ENOD40 | + | 1 | Repetitive | − | − | − |
026B19 | AAT-P2 | + | 1 | Single | − | 040A13 | + |
030F02 | Random | NI | NI | Single | 11 | KB424747.1, KB436872.1 | − |
033G07 | ENOD40 | + | 1 | Single | − | 131P18 | + |
036L23 | GS1 | + | NI | Single | 11 | AOCW01050906.1, KB412904.1 | + |
038J06 | NOD45 | + | 1 | Single | 9 | 138H12 | + |
040A13 | AAT-P2 | + | 1 | Single | − | 040A13 | + |
047P22 | GS1 | + | NI | Single | 4 | KB430042.1, KB405354.1 | + |
051D03 | NOD26 | + | 1 | Single | 6 | 127N17 | + |
056N03 | Random | NI | NI | Single | 11 | KB427843.1, KB423413.1 | − |
058D23 | ENOD40 | + | 1 | Repetitive | − | − | − |
058G22 | AAT-P2 | + | 1 | Single | − | − | − |
059A05 | ENOD40 | + | 1 | Repetitive | − | − | − |
059F07 | ENOD40 | + | 2 | Single | 6 | 059F07 | + |
059J08 | GS1 | + | NI | Single | 9 | AOCW01158579.1, KB441622.1 | + |
061F03 | NOD45 | + | 1 | Single | 9 | 138H12 | + |
064J15 | PEPC | + | NI | Repetitive | 13 | − | − |
066I06 | NOD26 | + | 3 | repetitive | − | − | − |
067C07 | PEPC | + | NI | Single | 19 | KB415203.1, KB435497.1 | + |
067J14 | NOD45 | + | 1 | Single | − | 138H12 | + |
068H10 | ENOD40 | + | 3 | Single | 18 | 068H10 | + |
070A03 | NOD45 | − | − | single | − | AOCW01153660.1, KB407058.1 | − |
076D23 | AS | + | NI | Single | 14 | AOCW01093208.1, AOCW01130134.1 | + |
081H12 | ENOD40 | + | 1 | Repetitive | − | − | − |
083F23 | PEPC | + | NI | Single | 19 | KB421397.1, KB435616.1 | + |
084A06 | AAT-P2 | − | NI | Single | 3 | KB431697.1 | − |
084H02 | ENOD40 | + | 1 | Repetitive | − | − | − |
085K17 | ENOD40 | + | 1 | Repetitive | − | − | − |
087F06 | NOD26 | + | 2 | Single | 3 | 087F06 | + |
087N22 | GS1 | + | NI | Single | 16 | KB421253.1 | − |
088H05 | ENOD40 | + | 1 | Repetitive | − | − | − |
089G04 | ENOD40 | + | 1 | Repetitive | − | − | − |
095N22 | ENOD40 | − | − | Repetitive | − | − | − |
097D16 | ENOD40 | − | − | Single | 11 | KB437521.1, AOCW01159605.1 | + |
102A04 | ENOD40 | − | − | Single | 13 | 102A04 | + |
103E24 | ENOD40 | − | − | Repetitive | − | − | − |
114O14 | AAT-P2 | + | 1 | Single | 3 | 040A13 | + |
122B05 | NOD45 | + | 1 | Single | − | 138H12 | + |
123D01 | NOD26 | + | 3 | Repetitive | 19 | − | − |
127N17 | NOD26 | + | 1 | Single | 6 | 127N17 | + |
130E19 | ENOD40 | + | 1 | Repetitive | − | − | − |
131K15 | PEPC | + | NI | Single | 5 | KB410794.1, KB422665.1 | + |
131K22 | AAT-P2 | − | − | Single | 4 | KB423564.1, KB405354.1 | + |
131P18 | ENOD40 | + | 1 | Single | 17 | 131P18 | + |
132P08 | ENOD40 | + | 1 | Single | − | 131P18 | + |
137N08 | AAT-P2 | + | 1 | Single | 3 | 040A13 | + |
138D07 | NOD45 | + | 1 | Single | − | 138H12 | + |
138H12 | NOD45 | + | 1 | Single | 9 | 138H12 | + |
103O20 | Centromere | NI | NI | Centromere | − | − | − |
134A23 | Centromere | NI | NI | Centromere | − | − | − |
072O21 | rDNA | NI | NI | rDNA | 16 | − | − |
102E23 | rDNA | NI | NI | rDNA | 2 | − | − |
NLL narrow-leafed lupin linkage group, NI data not included