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. 2016 May 11;24:355–378. doi: 10.1007/s10577-016-9526-8

Table 1.

BAC clone characterization including BAC-FISH signal, genetic localization, and anchoring to scaffolds

BAC clone Target gene Verification by sequencing with target gene-based primers Target gene sequence variant FISH signal NLL Assigned sequence (BAC or scaffold) Synteny (based on assigned sequence)
002B02 Random Single 4 KB439932.1 +
004L17 NOD45 + 1 Single
008A17 NOD45 + 1 Single 138H12 +
008O20 ENOD40 + 1 Single 17 131P18 +
010I16 ENOD40 Repetitive
018I03 ENOD40 + 1 Single 131P18 +
020A06 Random NI NI Single 11 AOCW01152381.1, KB427843.1
024K15 ENOD40 + 1 Repetitive
026B19 AAT-P2 + 1 Single 040A13 +
030F02 Random NI NI Single 11 KB424747.1, KB436872.1
033G07 ENOD40 + 1 Single 131P18 +
036L23 GS1 + NI Single 11 AOCW01050906.1, KB412904.1 +
038J06 NOD45 + 1 Single 9 138H12 +
040A13 AAT-P2 + 1 Single 040A13 +
047P22 GS1 + NI Single 4 KB430042.1, KB405354.1 +
051D03 NOD26 + 1 Single 6 127N17 +
056N03 Random NI NI Single 11 KB427843.1, KB423413.1
058D23 ENOD40 + 1 Repetitive
058G22 AAT-P2 + 1 Single
059A05 ENOD40 + 1 Repetitive
059F07 ENOD40 + 2 Single 6 059F07 +
059J08 GS1 + NI Single 9 AOCW01158579.1, KB441622.1 +
061F03 NOD45 + 1 Single 9 138H12 +
064J15 PEPC + NI Repetitive 13
066I06 NOD26 + 3 repetitive
067C07 PEPC + NI Single 19 KB415203.1, KB435497.1 +
067J14 NOD45 + 1 Single 138H12 +
068H10 ENOD40 + 3 Single 18 068H10 +
070A03 NOD45 single AOCW01153660.1, KB407058.1
076D23 AS + NI Single 14 AOCW01093208.1, AOCW01130134.1 +
081H12 ENOD40 + 1 Repetitive
083F23 PEPC + NI Single 19 KB421397.1, KB435616.1 +
084A06 AAT-P2 NI Single 3 KB431697.1
084H02 ENOD40 + 1 Repetitive
085K17 ENOD40 + 1 Repetitive
087F06 NOD26 + 2 Single 3 087F06 +
087N22 GS1 + NI Single 16 KB421253.1
088H05 ENOD40 + 1 Repetitive
089G04 ENOD40 + 1 Repetitive
095N22 ENOD40 Repetitive
097D16 ENOD40 Single 11 KB437521.1, AOCW01159605.1 +
102A04 ENOD40 Single 13 102A04 +
103E24 ENOD40 Repetitive
114O14 AAT-P2 + 1 Single 3 040A13 +
122B05 NOD45 + 1 Single 138H12 +
123D01 NOD26 + 3 Repetitive 19
127N17 NOD26 + 1 Single 6 127N17 +
130E19 ENOD40 + 1 Repetitive
131K15 PEPC + NI Single 5 KB410794.1, KB422665.1 +
131K22 AAT-P2 Single 4 KB423564.1, KB405354.1 +
131P18 ENOD40 + 1 Single 17 131P18 +
132P08 ENOD40 + 1 Single 131P18 +
137N08 AAT-P2 + 1 Single 3 040A13 +
138D07 NOD45 + 1 Single 138H12 +
138H12 NOD45 + 1 Single 9 138H12 +
103O20 Centromere NI NI Centromere
134A23 Centromere NI NI Centromere
072O21 rDNA NI NI rDNA 16
102E23 rDNA NI NI rDNA 2

NLL narrow-leafed lupin linkage group, NI data not included