Table 2.
Characterization of FISH signals detected for SSR-specific probes
| SSR motif | L. angustifolius (2n = 40) | L. luteus (2n = 52) | L. albus (2n = 50) | T. aestivum (2n = 42) | N. tabacum (2n = 48) |
|---|---|---|---|---|---|
| DIG(ATCCTC)3 | Centromere | Centromere | ND | Signals on several chromosomes | ND |
| DIG(GATAG)3 | Centromere | Centromere | ND | Signals on several chromosomes, two dominant loci | ND |
| DIG(AGG)5 | Centromere | Centromere | ND | Signals on several chromosomes | ND |
| Btn(AG)8 | Very weak signals dispersed on all lupin chromosomes | ND | ND | Signals on two chromosome pairs | ND |
| Btn(CTCC)4 | Very weak signals dispersed on all lupin chromosomes | ND | ND | Signals on three chromosomes | ND |
| Btn(CC)8 | Very weak signals dispersed on all lupin chromosomes | ND | ND | Dominant signals on one chromosome pair, weak signals in two chromosome pairs | ND |
DIG digoxigenin-labeled, Btn biotin-labeled, ND none detected