Table 2.
Mean expression levels of the 4 selected miRNAs and the fold change between the two groups by deep sequencing in the testing set with 20 patients and qRT-PCR in the validation set with 77 patients
| miRNAs | Methods | Non-progressive Group mean (SD) | Progressive Group mean (SD) | Fold-change | P-value** |
|---|---|---|---|---|---|
| miR-129-2-3p | Deep Seq (n=20) | 13.28 (9.67) | 2.75 (2.78) | 0.21 | 0.0001 |
| qRT-PCR (n=77) | 6.81 (1.98) | 6.21 (1.42) | 1.36 | 0.361 | |
| miR-204-5p | Deep Seq (n=20) | 848.03 (1117) | 239.16 (537.9) | 0.28 | 0.007 |
| qRT-PCR (n=77) | 3.55 (1.83) | 2.88 (1.70) | 1.60 | 0.250 | |
| miR-208b-3p | Deep Seq (n=20) | 51.86 (58.46) | 132.73 (169.1) | 2.56 | 0.003 |
| qRT-PCR (n=77) | 6.24 (1.89) | 5.43 (1.99) | 1.73 | 0.049 | |
| miR-3065-5p | Deep Seq (n=20) | 14.03 (9.36) | 5.01 (3.47) | 0.36 | 0.005 |
| qRT-PCR (n=77) | 6.23 (1.78) | 6.61 (1.79) | 0.86 | 0.564 |
*Mean count for deep sequencing and mean normalized Ct value for qRT-PCR.
**
p-value is unadjusted for multiple comparisons and is based on two-sample t-test comparing the means of the normalized miRNA expression levels.