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. 2016 Jul 1;6(7):1563–1571.

Figure 1.

Figure 1

MiR-153 directly targets HECTD3 mRNA 3’UTR. A. Sequence of the 3’UTR region of HECTD3 with putative miRNA-binding sites (underlined) predicted by three algorithms: TargetScan, miRanda, and Pictar. B. Western blot analysis for HECTD3 protein expression level in MDA-MB-231 cells after transfection with eight miRNAs as indicated for 2 days. C. HECTD3 levels were quantified in panel B by Image J software. D. HEK293T cells were transfected with the HECTD3 3’UTR luciferase reporter and an internal control pRL-β-actin constructs combined with indicated miRNAs or control for 24 hours. Then the luciferase activities were determined with Dual-Luciferase Reporter System. E. Sequences of wild type and mutant miR-153-binding sites in the 3’UTR of HECTD3 mRNA. F. HEK293T cells were transfected with the HECTD3 3’UTR or HECTD3 3’UTR-MUT luciferase reporter and an internal control pRL-β-actin combined with miR-153 and control for 24 hours, and luciferase activities were measured.