Figure 2.

H₂O₂ hampered tube formation and migration of HUVECs. HUVECs were treated with 0, 100, 200, 300 μM of H₂O₂ respectively for 4 hours and then cultured for another 24 hours. A: (left) Representative images of tube formation in HUVECs, (right) quantitative analysis of tube length were represented as fold of control (×100 magnification). B: Migrated cells were stained and quantitative analysis of migrated cells was represented as fold of control. Values are mean ± SEM; n = 4, N.S. means no significant difference, *means P<0.05, **means P<0.01, vs. control. Scale bar indicated 100 μm. One-way ANOVA (Bonferroni post hoc test) was used.