Figure 4.

SRT1720 protected against H₂O₂-induced senescence and apoptosis of HUVECs. HUVECs were pre-treated with 0, 5, 10, 15, 20 μM SRT1720 respectively for 24 hours, followed by 300 μM H₂O₂ for additional 4 hours. (A) SA-β-gal staining was performed and senescent cells were stained with blue color, the ratio of SA-β-gal positive cells was calculated per group. (B) An analysis of apoptosis by Hoechst 33258 Staining, Quantitative analysis was represented as the apoptotic cells in the total cells per field. Values are mean ± SEM; n = 4, N.S. means no significant difference, *means P<0.05, **means P<0.01, vs. control group. Scale bar indicated 100 μm in (A) and 50 μm in (B). One-way ANOVA (Bonferroni post hoc test) was used.