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Circulating Tfh cells in HC, HepB, and HCC subjects. A. Circulating Tfh cells are identified as CD4+CXCR5+ T cells in the representative gate. PBMCs were stained with CD3, CD4 and CXCR5 mAbs and Aqua dead cell stain for 30 min at 4°C. B. Frequencies of circulating Tfh cells in healthy control (HC), chronic HBV-infected (HepB), and HBV-related hepatocellular carcinoma (HCC) patients, stained directly ex vivo. Mean ± SD.
