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Anchorage-independent colonies growth of MKN-45 and BGC-823 cells stably silenced for Btk was shown. Cells were plated in soft agar. Two weeks later, colonies were stained with crystal violet and scored. Data are from three experiments performed in duplicate. The colonies formed in soft agar from the two respective silencing designs were photographed after two weeks. Data are the mean ± SD from 3 independent experiments. Statistical differences obtained at **P < 0.01.
