Figure 5.

Ibrutinib as a chemo-sensitizer. A. Cells were seeded at 5000 cells/well in 96-well plates overnight and co-treated with ibrutinib (2 μM) and DTX (2 ng/ml). The cell viability was measured using MTT assay after 24 h, 48 h and 72 h. B. The apoptotic effect of ibrutinib) in combination with DTX to MKN-45 and BGC-82 cells was further measured using Annexin-V and PI staining and flow cytometry. Data are the mean ± SD from 3 independent experiments. Statistical differences obtained at *P < 0.05, **P < 0.01. ##P <0.05, combination compared to DTX.