Figure 3.

Erianin induces T47D cells cycle arrest at G2/M phase. A. Erianin induces T47D cells cycle arrest. Cells were treated with graded concentrations of erianin (0 to 160 nM) for 48 and 72 hours. Propidium iodide staining was done to determine the DNA content. B. The percentage of cells in different phases of cell cycle was analyzed by flow cytometry. C. qPCR analysis for CDK family expression in erianin-treated cells. Data represent the mean ± SD (n=3), *P<0.05, **P<0.01, ***P<0.001 compared to control cells.