Figure 4.

PTEN is targeted by miRNA-152-3p in H9C2 cells. (A) PTEN mRNA level is regulated by miRNA-152-3p. H9C2 cells were transfected with miRNA-152-3p mimic, miRNA-152-3p inhibitor, or miRNA-scramble control and then PTEN mRNA was analyzed using qPCR. Each bar represents data from at least 3 repeated experiments. Significant differences between groups: *P < 0.05. (B, C) Western blotting analysis of PTEN expression in H9C2 cells transfected with miR-152-3p mimic or scramble control (B) or miR-152-3p inhibitor or scramble control (C).