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. 2016 Jun 22;35(15):1656–1676. doi: 10.15252/embj.201694401

Figure EV1. C9orf72 regulates the initiation of autophagy.

Figure EV1

  1. HEK293 cells treated with non‐targeting (Ctrl) or C9orf72 siRNA were incubated with vehicle (Ctrl), Torin1, BafA1, or BafA1 + Torin1 for 3 h, and levels of LC3‐I and II were determined on immunoblots. Levels of LC3‐II were normalized against α‐tubulin and are shown relative to the BafA1‐treated sample (mean ± SEM; one‐way ANOVA with Fisher's LSD test: ns, not significant; *P ≤ 0.05, ****P ≤ 0.0001; N = 3 experiments). These data are also shown in Fig 1C.
  2. HEK293 cells treated with non‐targeting (Ctrl) or C9orf72 siRNA were incubated with vehicle (Ctrl), rapamycin, BafA1, or BafA1 + rapamycin for 6 h, and levels of LC3‐I and II were determined on immunoblots. Levels of LC3‐II were normalized against α‐tubulin and are shown relative to the BafA1‐treated sample (mean ± SEM; one‐way ANOVA with Fisher's LSD test: ns, not significant; **P ≤ 0.01, ***P ≤ 0.001; N = 3 experiments). These data are also shown in Fig 1D.