Figure EV2. The LD proteins Dga1 and Yeh1 are degraded by the ERAD ubiquitin ligase Doa10.
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AThe degradation of Dga1‐GFP was analyzed in cells with the indicated genotype as described in Fig 1A. A plasmid‐borne GFP‐Dga1 expressed from the constitutive ADH1 promoter was used. The graph shows the average of two independent experiments; error bars represent the standard deviation.
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BThe degradation of Yeh1‐3HA was analyzed in cells with the indicated genotype as described in Fig 1A. A plasmid‐borne Yeh1‐3HA expressed from the endogenous promoter was used. The graph shows the average of two independent experiments; error bars represent the standard deviation.
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C, DThe degradation of Dga1‐GFP was analyzed in cells with the indicated genotype as described in Fig 1A. The graphs show the average of two independent experiments; error bars represent the standard deviation.