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. 2016 Jun 23;35(15):1707–1719. doi: 10.15252/embj.201694327

Figure 5. Interactions of FRH with RNA and ADP.

Figure 5

  1. 3.8 Å resolution mFo‐DFc omits map density (green, calculated with sharpened B‐factors and contoured at 2.0σ), reveals the presence of ADP and a short segment of RNA bound to FRH.
  2. 3.8 Å resolution 2mFo‐DFc calculated without ADP or RNA contributing to the model. Green electron density shown for ADP and RNA (0.6σ); orange density shown for surrounding protein (0.6σ).
  3. Comparison of Mtr4 and FRH ADP binding (FRH in cyan with ADP, RNA, and F179 in orange bonds; Mtr4 in green with ADP, RNA, and F148 in yellow bonds).
  4. Unwound DNA from Hel308 (pdb 2PR6; magenta, DNA in orange bonds) overlaid in FRH with RNA and ADP (FRH in cyan with yellow RNA and cyan ADP in stick) after superposition of the respective RecA domains. See also Appendix Fig S4. The superposition indicates that the KOW module is positioned to interact with double stranded RNA that is being unwound by the RecA and DHSCT domains.