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. 2016 Aug 2;7:107. doi: 10.3389/fendo.2016.00107

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Copyright © 2016 Lee, Tong, Duan, Foong, Musaitif, Yamazaki and Jefcoate.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of basal and induced expression of StAR by PKA-resistant SIK1–S577A–GFP. (A) Nuclear location of SIK1–S577A–GFP with and without Br-cAMP. (B) Suppression of Br-cAMP induction (180 min) of StAR RNA at the loci or in the cytoplasm in cells that transfected with SIK1–S577A–GFP versus GFP. (C) Quantitative expression in transfected Y-1 cells versus neighboring untransfected cells (a, image #1 and image #2). Associated mRNA intensity in 20 transfected cells versus 6 adjacent untransfected cells (b).