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. 2016 Aug 2;7:107. doi: 10.3389/fendo.2016.00107

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Copyright © 2016 Lee, Tong, Duan, Foong, Musaitif, Yamazaki and Jefcoate.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Suppressive effects of Tis11b in both Y-1 and MA-10 cells. (A) Induction of TIS11b in Y-1 and MA-10 cells by 1 mM Br-cAMP, measured by q-PCR (34). (B) Schematic for the suppression of TIS11b using siRNA and shRNA in the indicated cell lines. (C) Time course for the selective stimulation of 3.5-kb StAR mRNA following the suppression of Tis11b (34). (D,E) Effects of Tis11b (shRNA) suppression on the dose–response stimulation of StAR mRNA levels. (F,G) Effects of Tis11b (cell line) suppression on the dose–response stimulation of Tis11b mRNA and StAR protein levels.