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. 2016 Aug 2;6:30903. doi: 10.1038/srep30903

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Two pre-iPSCs cell lines, pre-2-2 and pre-3, were converted to iPSCs with Vc and indicated treatments. Numbers of GFP⁺ colonies were counted. (a,b) Retrovirus encoding sh-LSD1 and LSD1 were used for the conversion of pre-2-2 ((a), n = 6) and pre-3 ((b), n = 6). (c,d) T20 and GSK2879552 (2 μM) were used for the conversion of pre-2-2 ((c), n = 6) and pre-3 ((d), n = 6). (e,f) The effects of T20 on pre-iPSCs conversion were determined in pre-2-2 ((e), n = 6) and pre-3 ((f), n = 6) with reduced Vc concentrations. (g) Pre-2-2 were pre-treated with PBS (control), Vc and T20 from Day 0 to Day 4, and then treated with Vc or Vc+T20 from day 5 to Day 11. GFP⁺ colonies were counted. (h) Retrovirus encoding sh-Luc or sh-Hif1α was used with or without T20, and Rot was used with retrovirus encoding Flag or LSD1 during iPSCs generation. GFP⁺ colonies were counted.Two-way ANOVA with Bonferroni’s test as a post-hoc test was used.