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. 2016 Aug 2;2:16029. doi: 10.1038/celldisc.2016.29

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Copyright © 2016 The Author(s)

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Albino regulates expression of tetrapyrrole biosynthetic enzymes in planarian. (a) Expression fold changes of tetrapyrrole biosynthetic enzymes upon Albino RNAi. Shown are averages of three independent experiments; error bars=s.d. (b) WISH for tetrapyrrole biosynthetic enzymes in worms upon control or Albino RNAi, indicating the reduction of tetrapyrrole biosynthetic enzyme expressions. WISH samples were collected 7 days post fourth RNAi. Scale bar: 500 μm. (c) Representative double FISH results of ALAD, ALAS and PBGD with Albino in wild-type animals. Scale bar: 20 μm. Images are single confocal sections. (d) Representative double FISH results of ALAD and ALAS with PBGD in wild-type animals. Scale bar: 20 μm. Images are single confocal sections. (e) ISH of either wild-type worms or worms received 9 days of continuous direct light for Albino, PBGD, ALAD and ALAS.