Figure 4. Roles of Ltn1 in protein levels and of Dom34 in the ER protein import upon expression of nonstop ER membrane proteins.

Whole cell extracts prepared from the indicated yeast cells with expression of the indicated nonstop or stop membrane proteins from the GPD1 promoter were analyzed by SDS-PAGE and immunoblotting using the indicated antibodies. Asterisks indicate tRNA attached forms. m, mature protein; p, precursor form.