Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Aug 2;6:30795. doi: 10.1038/srep30795

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) Cell extracts were prepared from ski7∆dom34∆ cells expressing the indicated nonstop proteins from the GPD1 promoter by vortexing with glass beads, solubilized with 1.0% digitonin and then subjected to co-immunoprecipitation using anti-FLAG agarose beads. The immunoprecipitated proteins were analyzed by SDS-PAGE and immunoblotting. Asterisks, closed dots, and open dots indicate IgG bands, tRNA-attached forms, and tRNA-dissociated forms, respectively. (B,C) The indicated cells expressing Pho8-FLAG-ns from the GPD1 promoter were harvested at the indicated times after the addition of 200 μg/ml of cycloheximide. The cell extracts were prepared and analyzed as in (A). The amounts of Sec61 for the cells right after the addition of cycloheximide were set to 100%. Error bars represent SD (n = 3).