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. 2016 Aug 2;6:30004. doi: 10.1038/srep30004

Figure 3. Effect of IL-6 and LPS on NF-κB activation and transcription of pro-inflammatory and antimicrobial genes in B cells.

Figure 3

(a) Translocation of NF-κB to the nucleus in IgM+ splenocytes following stimulation with LPS (100 μg/ml) or IL-6 (200 ng/ml) for 24 h. The mean percentages of NF-κB translocation in total cells (upper panel) and IgM+ B cells (lower panel) from six independent experiments are shown. **P < 0.01, ***P < 0.001. (b) Spleen leukocytes were incubated with IL-6, LPS or control media alone for 24 h at 20 °C. The effect of IL-6 and LPS on the transcription of IL-1β1, IL-8, TNF-α3, cathelicidin-1 (CATH1), CATH2 and hepcidin was then studied in sorted IgM+ B cells from these cultures. The relative transcript expression (mean + standard deviation) of 6 independent experiments is shown. *P < 0.05, **P < 0.01, ***P < 0.001.