Figure 6. IL-6 has no effect on the phagocytic capacity of IgM⁺ B cells.

Splenocytes were cultured in the presence of IL-6 (200 ng/ml) or LPS (100 μg/ml) for 24, 48 or 72 h at 20 °C. Non-stimulated controls were also included. After the different incubation periods, cells were exposed to fluorescent beads for a further 3 h at 20  °C. Non-ingested beads were removed by centrifugation over a cushion of 3% (w/v) BSA in PBS supplemented with 4.5 (w/v) D-glucose (Sigma). (a) Data are shown as mean percentage of phagocytic IgM⁺ B cells (left) or Mean fluorescence intensity (MFI) (right) + standard deviation from six independent fish. *P < 0.05, **P < 0.01. (b) Representative dot plots for each condition.