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. 2016 Aug 2;6:30811. doi: 10.1038/srep30811

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Copyright © 2016, The Author(s)

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(a) Relative mRNA expression of L3MBTL4 in the blood vessels from wild type rats (WTs) and transgenic rats (TGs) (n = 7 each group). (b) L3MBTL4 protein levels in the vasculature are measured by western blot analysis (n = 7 per group). (c) Representative immunofluorescence images of L3MBTL4 (red) and α-actin (green) in the vessels from WTs and TGs. Nuclei are stained with DAPI (blue). Scale bars are 50 μm. n = 3 independent experiments. (d–h) Systolic blood pressure (SBP), diastolic blood pressure (DBP), mean blood pressure (MBP), heart rate (HR) and body weight (BW) of WTs and TGs (n = 8 each group). (j,k) Quantitative analysis of left ventricle + septum weight/body weight (LV + SW/BW), right ventricle weight/body weight (RVW/BW) and heart weight/body weight (HW/BW) ratios between groups (n = 6 each group). *p < 0.05, **p < 0.01, ***p < 0.001 compared to WT. All data represent mean ± s.e.m.