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. 2016 Aug 2;6:30805. doi: 10.1038/srep30805

Figure 3. Rescue of Rbfox1-iso1-knockdown-induced migration defects.

Figure 3

(a) Characterization of an RNAi-resistant version of Rbfox1, mRbfox1-iso1R. pCAG-Myc-mRbfox1-iso1 (iso1) or –iso1R was cotransfected into COS7 cells with pSuper-H1.shLuc (Control) or pSuper-mRbfox1-iso1#1. After 48 h, cells were harvested and subjected to western blotting with anti-Myc. Anti-Sept11 was used for loading control. Relative band intensity was also shown. (b) pCAG-EGFP was coelectroporated with pSuper-mRbfox1-iso1#1 together with pCAG vector (−) or pCAG-Myc-mRbfox1-iso1R into cerebral cortices at E14.5. After fixation at P3, analyses were performed as in Fig. 2a. Scale bar, 100 μm. (c) Quantification of the distribution of GFP-positive neurons in distinct parts of the cerebral cortex (bin 1–5, and IZ) for each condition shown in (b). Error bars indicate SD (n = 3); **p < 0.01, *p < 0.05 by Tukey-Kramer LSD.